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rotofor device  (Bio-Rad)


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    Bio-Rad rotofor device
    Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by <t>Rotofor</t> free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
    Rotofor Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rotofor+device/pmc03407172-188-9-11?v=Bio-Rad
    Average 90 stars, based on 1 article reviews
    rotofor device - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles"

    Article Title: Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0042064

    Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by Rotofor free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
    Figure Legend Snippet: Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by Rotofor free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.

    Techniques Used: Filtration, Centrifugation, Concentration Assay, Gradient Centrifugation, Activity Assay, Electron Microscopy



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    Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by <t>Rotofor</t> free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
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    Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by <t>Rotofor</t> free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
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    Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by <t>Rotofor</t> free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.
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    Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by Rotofor free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.

    Journal: PLoS ONE

    Article Title: Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles

    doi: 10.1371/journal.pone.0042064

    Figure Lengend Snippet: Exosomes were collected from the spent medium of the medulloblastoma cell line D283MED by filtration and differential centrifugation. Vesicles were analyzed by dynamic light scattering (DLS) ( A, left ) and nanoparticle tracking with a NanoSight device ( A, right ) for vesicle diameters and concentration. Sizes at identified peaks are listed. Accounting for dilution, exosome concentration in this case was 2.84×10 8 particles/ml. In ( B ), D283MED exosomes were subjected to density-gradient centrifugation thru a 0%–60% Opti-Prep step gradient; fractions were collected and densities determined. Fractions containing exosomes were identified by acetylcholinesterase (AChE) activity and electron microscopy (micrographs in inset above peak fractions 8 and 9; bar = 100 nm). Exosomes were also fractionated by Rotofor free-solution isoelectric focusing ( C ) as described in the . Fractions were harvested and the pH of each was determined. Exosome-containing fractions were again identified by AChE activity.

    Article Snippet: Free-solution isoelectric focusing was performed as described using a Rotofor device (Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Filtration, Centrifugation, Concentration Assay, Gradient Centrifugation, Activity Assay, Electron Microscopy